Kindling, one of the most used models of experimental epilepsy is based on daily electrical stimulation in several brain structures. We concluded that this novel RK protocol introduces a new variant of the chronic epileptogenesis models in freely moving rats, which is faster, highly reproducible and causes minimum cell damage with respect to that observed in other experimental models of epilepsĪ new rapid kindling variant for induction of cortical epileptogenesis in freely moving ratsĠ Centro de Neurobiología y Plasticidad Cerebral, Facultad de Ciencias, Instituto de Fisiología, Universidad de Valparaíso, Valparaíso, Chileġ Laboratorio de Neurobiología y Células Madre, Departamento de Biología Celular, Universidad de Concepción, Concepción, Chile
RK protocol induced convulsive states similar to SK protocols but in 3 days, with persistently lowered threshold to seizure induction and epileptogenic-dependent cellular changes in amygdala projection areas. Moreover, we assessed the effectiveness of RK protocol and the establishment of epilepsy by evaluating cellular alterations of hippocampal slices from kindled rats. The progression of epileptic activity was evaluated in freely moving rats through EEG recordings from cortex and amygdala, accompanied with synchronized video recordings. This new protocol consists of basolateral amygdala (BLA) stimulation with 10 trains of biphasic pulses (10s 50Hz) per day with 20 minutes-intervals, during 3 consecutive days, using a subconvulsive and subthreshold intensity, which guarantees tissue integrity. Here, we developed a new RK protocol in order to generate an improved model of temporal lobe epilepsy (TLE) which allows gradual progression of the epilepsy as well as obtaining an epileptic hippocampus, thus avoiding direct surgical manipulation and electric stimulation over this structure. However, the cellular changes observed in these rapid protocols, such as astrogliosis and neuronal loss, could be due to experimental manipulation more than to epileptogenesis-related alterations. Unlike the classic or slow kindling protocols (SK), the rapid kindling types (RK) described until now require continuous stimulation at suprathreshold intensities applied directly to the same brain structure used for subsequent electrophysiological and inmunohistochemical studies, usually the hippocampus. Astroglial Ca2+-Dependent Hyperexcitability Requires P2Y1 Purinergic Receptors and Pannexin-1 Channel Activation in a Chronic Model of Epilepsy Neuronal plasticity associated with mesial temporal lobe epilepsy: insights form human and animal model studiesĪstroglial Ca2+-Dependent Hyperexcitability Requires P2Y1 Purinergic. Neuronal plasticity associated with mesial temporal lobe epilepsy: insights. Suppression of kindling epileptogenesis by adenosine releasing stem cell-derived brain implants Suppression of kindling epileptogenesis by adenosine releasing stem cell. Occurrence of Epilepsy at Different Zeitgeber Times Alters Sleep Homeostasis Differently in Rats Occurrence of Epilepsy at Different Zeitgeber Times Alters Sleep Homeostasis.
The Spatiotemporal Dynamics of Phase Synchronization during Epileptogenesis in Amygdala-Kindling Mice The Spatiotemporal Dynamics of Phase Synchronization during Epileptogenesis. Z944, a Novel Selective T-Type Calcium Channel Antagonist Delays the Progression of Seizures in the Amygdala Kindling Model Z944, a Novel Selective T-Type Calcium Channel Antagonist Delays the. A Transient Upregulation of Glutamine Synthetase in the Dentate Gyrus Is Involved in Epileptogenesis Induced by Amygdala Kindling in the Rat A Transient Upregulation of Glutamine Synthetase in the Dentate Gyrus Is.
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